Large Qligonucleotide Sequences in Wheat Germ Phenylalanine Transfer Ribonucleic Acid
نویسندگان
چکیده
The nucleotide sequence analysis of tRNA has required (a) identification of fragments isolated from complete digestion with pancreatic RNase and RNase T1 and, in addition, (b) the analysis of large oligonucleotide fragments produced by partial digestion with RNase T1 (l-4) and, in some cases, partial digestion with pancreatic RNase (5). In continuation of the work reported in the preceding paper (6), this paper reports in detail the conditions of controlled cleavage of tRNAPh” ’ with RNase T1 and pancreatic RNase and the separation and analysis of the large oligonucleotide fragments. The nucleotide sequence of tRNAPh” is deduced from overlaps among the large oligonucleotides produced.
منابع مشابه
Amino acid incorporation and aminoacyl transfer in a wheat embryo system.
An amino acid-incorporating system has been obtained from ungerminated wheat embryos. This system has very low endogenous activity, but phenylalanine incorporation can be stimulated by the addition of polyuridylic acid. The poly U-stimulated system is similar to those from other sources in that it requires ribosomal particles, supernatant fluid, soluble ribonucleic acid, adenosine triphosphate,...
متن کاملPrimary structure of wheat germ phenylalanine transfer RNA.
The complete nucleotide sequence of wheat germ phenylalanine transfer RNA (tRNA(Phe)) is presented. This RNA, which is an acceptable substrate for yeast phenylalanine tRNA synthetase, has a structure very similar to that of yeast tRNA(Phe). Only 16 of the 76 nucleotides are different, and all but two of the nucleotide changes are located in regions that are doublestranded in the cloverleaf mode...
متن کاملProflavine inhibition of protein synthesis.
Prcflavine, in the range of 5 x 1OU5 M, markedly inhibited prctein synthesis in a subcellular rat liver system. With native messenger ribonucleic acids, leucine incorporation was inhibited more than phenylalanine incorporation. In the presence of polyuridylate, however, phenylalanine incorporation was inhibited more than leucine incorporation. Reversal studies indicated that excess transfer rib...
متن کاملThe Effect of Nucleic Acids and of Carbohydrates on the Formation of Streptolysin
1. Ribonucleic acid of yeast causes the formation of a potent hemolysin in broth cultures of Streptococcus pyogenes. 2. The hemolysin whose formation is induced by yeast ribonucleic acid appears to be identical with streptolysin S. 3. Desoxyribonucleic acid, products of acid or alkaline hydrolysis of ribonucleic acid, or many other substances tested, fail to produce a similar effect. 4. Digesti...
متن کاملPleiotropic phenotype of an Escherichia coli mutant lacking leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase.
A mutant of Escherichia coli that lacks leucyl-, phenylalanyl-transfer ribonucleic acid-protein transferase had diminished activities of L-phenylalanyl-transfer ribonucleic acid synthetase and tryptophanase, grew faster than its parent with aspartic acid as the sole nitrogen source, accumulated higher levels of enterochelin in the medium during iron limitation, and exhibited an abnormal morphol...
متن کامل